PGD₂ stimulation of actin polymerization in eosinophils.

Purified eosinophils were incubated for 20 seconds at 37°C with PGD₂ (●; n = 5), 5-oxo-ETE (○, 5o-ETE; n = 5), PGF_2α (▴; n = 4), and PGE₂ (▿; n = 3). The cells were then permeabilized and stained with fluorescently labeled phallacidin. F-actin levels were measured by flow cytometry. The results are expressed as percentage increases (± SE) in F-actin content above basal levels.