Selective stimulation of CD11b expression on eosinophils by PGD2.
CD11b expression was measured by flow cytometry on eosinophils (A) and neutrophils (B), which were distinguished from one another and from other leukocytes on the basis of side scatter (SSC), a measure of granularity, and staining with PE-labeled anti–VLA-4. Eosinophils and neutrophils were gated out as shown by the dot plots in the insets. Eosinophils are shown by the black dots in the inset to panel A, whereas neutrophils are shown by the black dots in panel B. CD11b expression by the gated cells was measured by means of FITC-labeled anti-CD11b. Histograms are shown for cells stained with an FITC-labeled isotype control antibody (black) and with FITC-labeled anti-CD11b for unstimulated cells (control; light gray shading), PGD2(heavy line), and 5-oxo-ETE (5o-ETE, dark gray shading).
