Effect of immobilized Delta^ext-myc on differentiation of CD34⁺ cell-derived macrophage/dendritic precursors.

Cells were generated from CD34⁺ cell-derived CD1a⁻CD14⁺ cells cultured for 8 days with GM-CSF, TNF-α, and either 1 μg/mL Delta^ext-myc or control medium. All tissue culture wells were coated with anti-myc antibody, 9E10 F(ab′)₂, to attach myc-containing Delta^ext-myc to the plastic surface. (A) Phase contrast microscopy of cultured cells (original magnification × 100). (B) CD14 and CD1a expression profile of cultured cells. The x-axis represents log fluorescence intensity for CD14 (My4) and the y-axis represents log fluorescence intensity for CD1a. One representative experiment of 4 is shown. (C) MLR-stimulatory capacity of cells from cultures containing 1 μg/mL Delta^ext-myc (●) or control medium (○). Values are the mean ± SD obtained from triplicate cultures. One representative experiment of 3 is shown. (D) Cells were stained with anti-RelB antibody (see “Materials and methods”) and nuclei were counterstained with DAPI (original magnification × 40).