American Society of Hematology

Fig. 6.

$Fig. 6. Affinity chromatography of citrated normal human plasma on anticlusterin-Sepharose. / (A) Protease activities of normal plasma (a), of the first 5 breakthrough fractions (b1-b5), and of the first 2 fractions eluted with NaSCN (c1, c2). vWF-cp passed undelayed through the anticlusterin column, although clusterin was completely sequestered, as shown by the dot blot analysis (B). There was no vWF-cp released from the column together with clusterin during elution with NaSCN.$

Affinity chromatography of citrated normal human plasma on anticlusterin-Sepharose.

(A) Protease activities of normal plasma (a), of the first 5 breakthrough fractions (b1-b5), and of the first 2 fractions eluted with NaSCN (c1, c2). vWF-cp passed undelayed through the anticlusterin column, although clusterin was completely sequestered, as shown by the dot blot analysis (B). There was no vWF-cp released from the column together with clusterin during elution with NaSCN.

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