American Society of Hematology

Fig. 5.

$Fig. 5. Affinity chromatography on anticlusterin-Sepharose of the crude protease preparation eluted from the IgG-AffiGel column with NaSCN. / (A) vWF-cp activity in the protease preparation before application onto anticlusterin column (a), in the breakthrough fraction (b), and in the NaSCN-eluted fraction (c). The activity was bound in the anticlusterin column and released during elution with NaSCN. (B) Dot blot analysis of the same 3 samples, using dilutions (dil) 1:2 to 1:32, shows complete clusterin removal on anticlusterin-Sepharose and its dissociation in the chaotropic medium.$

Affinity chromatography on anticlusterin-Sepharose of the crude protease preparation eluted from the IgG-AffiGel column with NaSCN.

(A) vWF-cp activity in the protease preparation before application onto anticlusterin column (a), in the breakthrough fraction (b), and in the NaSCN-eluted fraction (c). The activity was bound in the anticlusterin column and released during elution with NaSCN. (B) Dot blot analysis of the same 3 samples, using dilutions (dil) 1:2 to 1:32, shows complete clusterin removal on anticlusterin-Sepharose and its dissociation in the chaotropic medium.

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