Fig. 4.
Two-dimensional electrophoretic analysis of a pool of active fractions collected from the Sephacryl S-300 HR column.
The slowest migrating unreduced proteins (Mr 350 kd or greater) produced subunits of 170 and 120 kd after disulfide reduction, whereas the 70-kd band was reduced to 40 kd. Four protease bands in the Mr range of 110 to 150 kd are single-chain polypeptide chains.
