FACS analyses of donor-derived CD3⁻CD4⁻ CD8⁻ (TN) thymocytes.

Thymocytes from representative normal and BMT recipients on day 28 were stained with anti-CD3–fluorescein isothiocyanate, a cocktail of anti-CD4–phycoerythrin and anti-CD8–phycoerythrin, and anti-Thy1–APC antibodies to determine the frequency of TN thymocytes. Analyses of the Thy1-positive cells are shown, with the percentages of TN thymocytes indicated for each mouse.