Fig. 7.
Fig. 7. Effects of alanine-substituted FANCCmutations on STAT1 phosphorylation in normal JY cells. / Upper panel: JY alanine-substituted FANCC mutant cells (lanes 5-16) were exposed to 1 ng/mL IFN-γ for times indicated, and compared to JY cells (lanes 1 and 2) as well as JY/FANCC cells transduced with wild-type FANCC (lanes 3 and 4). Cell lysates were separated by SDS-PAGE in a 7.5% polyacrylamide gel and immunoblotted with an antibody to tyrosine phosphorylated STAT1. Lower panel: The blot described in the upper panel was stripped and reprobed with an antibody to total STAT1 protein. Reduced amounts of phosphorylated STAT1 in lanes 8 and 10 appear to be a result of reduced total STAT1 protein in this experiment. In all subsequent experiments this particular finding was not noted.

Effects of alanine-substituted FANCCmutations on STAT1 phosphorylation in normal JY cells.

Upper panel: JY alanine-substituted FANCC mutant cells (lanes 5-16) were exposed to 1 ng/mL IFN-γ for times indicated, and compared to JY cells (lanes 1 and 2) as well as JY/FANCC cells transduced with wild-type FANCC (lanes 3 and 4). Cell lysates were separated by SDS-PAGE in a 7.5% polyacrylamide gel and immunoblotted with an antibody to tyrosine phosphorylated STAT1. Lower panel: The blot described in the upper panel was stripped and reprobed with an antibody to total STAT1 protein. Reduced amounts of phosphorylated STAT1 in lanes 8 and 10 appear to be a result of reduced total STAT1 protein in this experiment. In all subsequent experiments this particular finding was not noted.

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