Expression of alanine-substituted FANCC mutant protein in PD4 cells.

PD4 cell lysates (100 μg each) were subjected to SDS-PAGE in a 10% polyacrylamide gel. PD4 alanine-substituted mutant cells (lanes 3-8) were compared to PD4 cells (lane 1), PD4/FANCC cells complemented with wild-type FANCC (lane 2), and PD4/neo cells transduced with vector alone (lane 9). The immunoblot was probed with rabbit polyclonal antiserum generated against the N-terminus of the FANCC protein.