Effects of alanine-substituted FANCCmutations on STAT1 phosphorylation in HSC536N cells.

Upper panel: The HSC536N FANCC mutant cells (lanes 5-16) were exposed to 1 ng/mL IFN-γ for times indicated and compared to HSC536N cells (lanes 1 and 2) as well as HSC536N/FANCC cells complemented by transduction with wild-type FANCC (lanes 3 and 4). Cell lysates were separated by SDS-PAGE in a 7.5% polyacrylamide gel and immunoblotted with an antibody specific for STAT1 protein phosphorylated on Y701. Lower panel: The blot described in the upper panel was stripped and reprobed for total STAT1 protein.