FKN expression in lymph nodes.

FKN expression was tested by immunohistochemistry (A-C) and by immunofluorescence (D-H) in lymph nodes from 4 HIV-infected patients and 4 controls. (A) Two HEV cells in a T-cell zone of an HIV-uninfected patient. (B,C) T-cell zone in an HIV-infected patient. (D,G,H) Characterization of FKN-expressing cells in an HIV-infected patient, evidenced by a double-labeling experiment combining detection of FKN (red) and CD83 (D), CD123 (G), or CD79α (H) (green). Double-labeled cells appear in yellow. Single-labeling immunofluorescence experiments with the anti-FKN or the CD83 antibody are shown in panels E and F, respectively. → indicates DCs; ➤, HEV; *, plasma cell. Original magnification × 400 in panels B, D, and E and × 200 in other panels.