Intracellular expression of cytokines in BM DCs at different stages of cell differentiation.

(A) Detection of intracellular cytokines by flow cytometry in CD86⁻ and CD86⁺ BM DCs. CD11c⁺immunobead-sorted DCs were double labeled with FITC anti-CD86 and PE anticytokine mAbs. Figures within quadrants indicate percentages of cells. (B) Detection of TGF-β1 by ELISA in 24-hour culture supernatants of FACS-sorted CD86⁻ DCs and CD86⁺ DCs (2 × 10⁶ cells/well). Results are representative of 4 separate experiments.