Comparative RPA analysis of cytokine mRNA expression of BM DCs at different stages of cell differentiation.

mRNA isolated from FACS-sorted DCs was analyzed by RPA. Data are from a single experiment representative of 3 separate experiments. (B) Quantitative analysis of mRNA cytokine gene expression. Densitometric analysis of each lane was performed on scanned autoradiographs, and all values are expressed relative to corresponding housekeeping gene transcripts (L32). ■, CD11c⁺CD86⁻ DCs; ▪, CD11c⁺CD86⁺ DCs. Densitometric values were pooled from 3 separate experiments.