DUB-2 prolongs survival following cytokine withdrawal.
(A) Ba/F3β/tTA DUB-2 (clones 6 and 7) were incubated with or without tetracycline for 48 hours, stimulated with IL-2 for 4 hours, and incubated in cytokine-free medium for the indicated times. Cell death was monitored by trypan blue exclusion assay, and data expressed as percentage of cells excluding trypan blue. (B) Ba/F3β/tTA DUB-2 cells (clone 6, +/−Tet) were stimulated with IL-2 for 4 hours, and withdrawn from cytokine as above. At the indicated times, cells were stained with PI and analyzed by flow cytometry. Apoptotic cells correspond to cells with sub-G1 DNA content (M1). Numbers indicate the percentage of cells in the different phases of the cell cycle (M2: G0/G1; M3: S; M4: G2/M).
