DUB-2 enhances expression of STAT5-dependent genes.

Ba/F3β/tTA DUB-2 cells were grown with or without tetracycline for 48 hours, stimulated with IL-2 for 2 hours, and incubated in cytokine-free medium for the indicated times. Cells lysates were immunoprecipitated with anti-STAT5b or ERK2, and immunoblotted with antiphosphotyrosine (pY), antiphospho-ERK (p-ERK), anti-STAT5b, or anti-ERK2 antisera, as indicated. Whole cell lysates were also run and immunoblotted with antisera for phospho-AKT (p-AKT), AKT, DUB-2, Cis, c-Myc, and Bcl-x, as indicated.