American Society of Hematology

Fig. 1.

$Fig. 1. T lymphocytes binding the HLA-A2 NLVPMVATV (A2-NLV) tetramer are CD8bright and not CD8dim. / Following acquisition of a list mode data file containing 20 000 viable CD8+ lymphocytes, viable cells were selected on the basis of 7-AAD negativity (Gate 1). Among the viable mononuclear cells, CD8+ lymphocytes were selected on the basis of CD8 expression and a low sideward scatter signal (Gate 2). Lymphocytes fulfilling the criteria of Gates 1 and 2 are displayed in the figure. The percentage of A2-NLV–specific CD8+ T lymphocytes was assessed and expressed as a fraction of viable CD8+lymphocytes by using quadrant statistics. PE, phycoerythrin; APC, allophycocyanin.$

T lymphocytes binding the HLA-A2 NLVPMVATV (A2-NLV) tetramer are CD8^bright and not CD8^dim.

Following acquisition of a list mode data file containing 20 000 viable CD8⁺ lymphocytes, viable cells were selected on the basis of 7-AAD negativity (Gate 1). Among the viable mononuclear cells, CD8⁺ lymphocytes were selected on the basis of CD8 expression and a low sideward scatter signal (Gate 2). Lymphocytes fulfilling the criteria of Gates 1 and 2 are displayed in the figure. The percentage of A2-NLV–specific CD8⁺ T lymphocytes was assessed and expressed as a fraction of viable CD8⁺lymphocytes by using quadrant statistics. PE, phycoerythrin; APC, allophycocyanin.

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