Standard curves for quantification of BKV and ADV.

(A) Amplification plots of pB-VP1 for the construction of a standard curve. Amplification plot for reactions with known starting amounts of pB-VP1 (0.5-5000 fg plasmid DNA, denoted by boxes next to the corresponding curves). Gray curves denote no template control reactions. Cycle number is plotted against change in normalized reporter signal (ΔRn). For each reaction, the fluorescence signal of the reporter dye (FAM) is divided by the fluorescence signal of the passive reference dye (ROX), to obtain a ratio defined as the normalized reporter signal (Rn). (B) Plot of standard curve of starting pB-VP1 amount against C_T. Black circles represent pB-VP1 standards as demonstrated in panel A. Gray circles represent patient samples. A standard curve is constructed for every assay.