Screening of anti–HSV antibody–producing cells.

Supernatants from the first round (A) and the second round (B) of limiting dilution using EBV-transformed LCLs established from the patient were collected and screened by a Herpes Simplex IgG detection kit as described in “Materials and methods.” OD at 450 nm was measured in both experiments. N, negative control; P, positive control. (C) Sequence analysis of the positive clone (clone 67-2) after the second round of limiting dilution, indicating that the clone was of host origin. An asterisk indicates the position of the paternal mutation.