Fig. 9.
![Fig. 9. Influence of sphingomyelinase treatment on ceramide metabolism and protein synthesis. / (A,B) HL-60/VCR cells, prelabeled with [3H]palmitic acid, were incubated with sphingomyelinase for 4 hours, and lipids were extracted and analyzed. (C) To measure protein synthesis, cells were incubated with sphingomyelinase for 1 hour, before addition of [3H]leucine (1.0 μCI/well) for 3 hours. Data points represent the mean ± SD (n = 3). SD bars are not shown (error < 5%). SM indicates sphingomyelin.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/6/10.1182_blood.v98.6.1927/7/h81811534009.jpeg?Expires=1724189354&Signature=0dk4LJ25~Wm2VEYF5gf7~yqxYUTszv1HBTK-IQ9JF-Pqyl2Elzje63FMJGwpRauE9Zmj8-0jjY0ALnIxy2HnKebNn2rQIjsXZky7XIpGBj5wP50MgzCgaoDLKtATxVkZrGKcHuaQb9sgKgIkuDQmTNdaQUlFyCwYmhfoPc~yDDwzFwjMccobiRi-mH4OVHaW9kpYMkoHkLFjtTDxTSAMWp~UxyPatSKT-guFRkB7CZwCqrN-KeAZhYpcBxY5n7ncW3VE2H1AJWvsD30PVOZ2f1a9QX3LmYjbJpgmBqUOehEVNeGU2rZNs5s~3xdnz5eMOVdW-9JGGnWYBI2uVrmFXw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Influence of sphingomyelinase treatment on ceramide metabolism and protein synthesis.
(A,B) HL-60/VCR cells, prelabeled with [3H]palmitic acid, were incubated with sphingomyelinase for 4 hours, and lipids were extracted and analyzed. (C) To measure protein synthesis, cells were incubated with sphingomyelinase for 1 hour, before addition of [3H]leucine (1.0 μCI/well) for 3 hours. Data points represent the mean ± SD (n = 3). SD bars are not shown (error < 5%). SM indicates sphingomyelin.
