Proteases cleaving rhuVCAM-1 accumulate in BM of G-CSF–mobilized mice.

rhuVCAM-1 was incubated for 15 minutes at 37°C in the presence of either PBS (Nil), BM extracts from 3 different mice injected for 6 days with saline (S1, S2, S3), or from 3 different mice injected for 6 days with G-CSF (G1, G2, G3), and analyzed by Western blot.