Expression of VCAM-1 is reduced in the BM of mice mobilized with G-CSF.

BM monolayers were stained with the mAb M/K2-7 (B-F) or with an isotypic control (A). BM were isolated from mice infused for 6 days with saline (A,B) or G-CSF (C,D). Panels E and F show VCAM-1 staining on frozen sections of spleens from mice infused for 6 days with saline (E) or G-CSF (F). Some of the megakaryocytes present in these BM samples are indicated with the letter “m”; the lymphoid areas of the spleen are indicated by “ly.” Each micrograph is representative of BM monolayers from 15 to 27 different mice in each group. The red color shows VCAM-1 staining (× 250 magnification).