TNF does not affect cell-cycle distribution of ex vivo–expanded CD34⁺ CB cells.

Cell-cycle status of freshly isolated and cultured (in KFT3 in the absence or presence of TNF for 6 days) CD34⁺ CB cells stained with anti–Ki-67 and 7-AAD (as described in “Materials and methods”). KFT3 cells expanded 58-fold in the absence of TNF and 44-fold in the presence of TNF. One representative experiment of 2 is shown.