TNF does not enhance apoptosis of ex vivo expanded progenitors.

CD34⁺CB cells cultured for 6 days in KFT3 in the absence or presence of TNF (20 ng/mL) were stained with anti-CD34–APC, annexin V–PE, and 7-AAD, as described in “Materials and methods.” Annexin V and 7-AAD staining was investigated on the total cell population and on CD34⁺-gated cells. KFT3-treated cells expanded 55-fold, whereas KFT3 + TNF-cultured cells expanded 35-fold. One representative experiment of 2 is presented.