Ex vivo expanded CD34⁺CD38⁻cells exposed to TNF have severely compromised in vivo multilineage reconstituting activity.

Multilineage human engraftment of a mouse transplanted with EEs of 5000 CD34⁺CD38⁻ cells cultured for 7 days in KFT3 as well as a mouse transplanted with EEs of 5300 CD34⁺CD38⁻ cells cultured under identical conditions except in the presence of TNF (20 ng/mL) is shown. Of all mice transplanted with TNF-cultured cells, this was the one that showed the highest level of human reconstitution, and the only one with both myeloid and lymphoid engraftment.