Fig. 1.
![Fig. 1. Effects of agonistic and antagonistic anti-Fas Abs on Jurkat cells. / Jurkat cells (20 000) were cultured in 100 μL RPMI 1640 with 10% FCS in the absence or presence of 1 μg/mL CH11 (agonistic anti-Fas Ab) and/or ZB4 (blocking anti-Fas Ab) at different concentrations as indicated. Following 24 hours incubation, each well was pulsed for 24 hours with 1 μCi [3H]TdR before being harvested and evaluated for [3H]TdR uptake (cpm) as described in “Materials and methods.” Each group was performed in triplicate and error bars represent SD.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/6/10.1182_blood.v98.6.1782/7/h81811540001.jpeg?Expires=1724239926&Signature=gtnrZpwj3d8~4hYi1cDYCdAna47UzC652CIp67v2-1vHtTYBfB0tNMmWVQ9BAJnU-GykzKrR3HmRUTdMDa6wDzIRGzh3LdVA-pn4Za5BeYXR4Y0rNcAh8OmXk7Leh4abf82lpRf98viVWVNsqavQb4C7Ywu5j0YEcGTkuhayd5kki8PpX1MP~qOUho8mytw4nyviCXZN0PspOq5XdwCtbQBprqyFXSxVAM3-zOS9YR~vyBUt0see0PLl3vgmnMk42YHr79fDTRiVCwRnz7IObSbdzu7ql1Y4jfJuy8U0NVraCykewlErpdCqeGAYY7VDMPEYzSCm8tFSokmupPQLkA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of agonistic and antagonistic anti-Fas Abs on Jurkat cells.
Jurkat cells (20 000) were cultured in 100 μL RPMI 1640 with 10% FCS in the absence or presence of 1 μg/mL CH11 (agonistic anti-Fas Ab) and/or ZB4 (blocking anti-Fas Ab) at different concentrations as indicated. Following 24 hours incubation, each well was pulsed for 24 hours with 1 μCi [3H]TdR before being harvested and evaluated for [3H]TdR uptake (cpm) as described in “Materials and methods.” Each group was performed in triplicate and error bars represent SD.
