The effect of LiCl on growth factor–deprived human erythroblasts.

(A) Cells on day 5 to 7 of culture were washed and returned to medium either with or without growth factors (Epo 2 U/mL and SCF 20 ng/mL) or, in the case of cells without growth factors, either with or without LiCl 20 mM (n = 5). The mean percentage ± SEM of apoptotic cells at 8 hours, as determined by annexin V binding, is indicated. (B) Cells deprived of growth factors were treated with various concentrations of LiCl as indicated. The percentage protection against apoptosis (± SEM) at 8 hours compared with that obtained with 20 mM LiCl is indicated (n = 4).