PS exposure in murine sickle cell bone marrow.

RBCs (A) were labeled with biotin–annexin V–PE-streptavidin and thiazole orange. Bone marrow (B) and spleen (C) cells were labeled with Ter119-PE, biotin–annexin V–allophycocyanin-conjugated–streptavidin, thiazole orange, and 7-AAD. Projected events were gated for intact thiazole orange staining (immature), non–7-AAD staining (nonnecrotic), erythroid (Ter119-labeled) cells. The quadrant marker was placed to distinguish between enucleated (left) and larger nucleated (right) cells and was annexin-labeled (top) from unlabeled cells (bottom). Graphs represent examples of data obtained from one mouse but typical for 5 mice.