Reduced RBC survival time of PS-exposing sickle cells.

Murine RBC population was biotinylated on day 0, and at the indicated time intervals, RBCs were sampled and double-labeled with PE-streptavidin and GFP–annexin V to assess the survival of PS-exposing cells. Data were fitted to A(t) = A0[1−(t/T)]exp^−kt, in which A(t) is the number of biotinylated RBCs at time (t), A0 is the initial number of biotinylated cells at t = 0, and T is the time of senescent death of RBC. Data are depicted for 6 different mice; the annexin V–binding population is indicated as filled circles representing the mean ± SD, whereas the biotinylated population is depicted as open symbols representing each individual mouse. Curve fits are indicated for the total population (solid line) and the annexin V–binding population (dashed line).