Effect of patients' anti-FIX IgGs on FVIII light chain binding to FIX.

The ability of various concentrations of mouse monoclonal anti-FIX antibody CLB-FIX 11 (■), mouse monoclonal anti-FIX antibody CLB-FIX 14 (○), and IgGs from patient 1 (▵), patient 2 (●), patient 3 (▴), patient 4 (▪) to inhibit thrombin-cleaved FVIII light chain binding to immobilized FIXa was investigated (see “Patients, materials, and methods”). The data represent mean values from 3 independent experiments.