![Fig. 5. Detection of transgene expression in E18 CMV-GMTagAU+ transgenic embryo and associated phenotypes. / (A) In situ hybridization using [35S]-labeled GM-CSF antisense riboprobe showing transgene expression foci in olfactory epithelium (oe) and gut (gu) (magnification × 2). (B) Transgene expression in olfactory epithelium at greater magnification. (C,D,G) Immunohistochemical detection of granulocytes with anti-Gr1 antibody (brown peroxidase staining) surrounding olfactory epithelium (C), lungs and the rib bone marrow (D, arrows and BM, respectively), and lung and rib bone marrow of nontransgenic embryo (G). (E,F,H,I) Detection of peroxidase-positive cells (black staining) in spleen (E) and gut (F) of transgenic compared to nontransgenic embryo (respectively, H and I); br indicates brain; BM, bone marrow; gu, gut; oe, olfactory epithelium; and sp, spleen (B, C, E, F, H, I, magnification × 100; D, G, magnification × 200). The expression pattern and the phenotype are representative of the 2 embryos analyzed.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/5/10.1182_blood.v98.5.1281/5/h81711446005.jpeg?Expires=1722367635&Signature=iwYv0YCEPyAUlZzhuyFjU7UsIAfbePkCDmrJixWNg4HpPF0RsrIZJeKz5xIeUOWZ3sRWs3YUIB9GQF8ajaNDILuzSQHntoLteXsZzj6KSl6gH43l7gDxT8zLpClSQV-mZ4lUt1FXHIEoPixFsN6DhT~Gkk~gRtAda~oHKMNC8XoLVOOs65tVw7BS~pz--A0EwY6b3o7o3rLTFnvMU-epK1SfQgfyRID6Q7kNwjOLwFjHqD3~f-cTOQ8YywV6qGBYuv0nkeh1ogRvNt6yoW4NLMJhVvtJ6t7G5xKQeCoWKkEoP~44LVFf3qrSARuNXXmkzgrLvoUbaGYHXzT3a3eVpw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of transgene expression in E18 CMV-GMTagAU+ transgenic embryo and associated phenotypes.
(A) In situ hybridization using [35S]-labeled GM-CSF antisense riboprobe showing transgene expression foci in olfactory epithelium (oe) and gut (gu) (magnification × 2). (B) Transgene expression in olfactory epithelium at greater magnification. (C,D,G) Immunohistochemical detection of granulocytes with anti-Gr1 antibody (brown peroxidase staining) surrounding olfactory epithelium (C), lungs and the rib bone marrow (D, arrows and BM, respectively), and lung and rib bone marrow of nontransgenic embryo (G). (E,F,H,I) Detection of peroxidase-positive cells (black staining) in spleen (E) and gut (F) of transgenic compared to nontransgenic embryo (respectively, H and I); br indicates brain; BM, bone marrow; gu, gut; oe, olfactory epithelium; and sp, spleen (B, C, E, F, H, I, magnification × 100; D, G, magnification × 200). The expression pattern and the phenotype are representative of the 2 embryos analyzed.
