![Fig. 3. Transgene expression. / Detection under dark field illumination (magnification × 6) of CMV-GMTag AU− (A) and AU+ (B) transgene expression in E14 embryo by in situ hybridization using [35S]-labeled GM-CSF antisense riboprobe; (C) nontransgenic embryo. The white dots observed in the CMV-GMTag AU− sample correspond to dispersed transgenic mRNA-containing cells. White lines appearing in the CMV-GMTag AU+ and nontransgenic samples correspond to artifacts due to dust and manipulation. (D-G) Detection under bright field illumination of CMV-GMTagAU− transgene expression (black dots) (D) and expression foci at greater magnification (× 100) in the choroid plexus (E), the olfactory epithelium (F), and heart (G). (H) Staining of the heart by hemalum-eosin to show transgene expression by cells in clusters in blood vessels (arrows); br indicates brain; cp, choroı̈d plexus; he, heart; oe, olfactory epithelium; and ve, ventricule. These expression patterns are representative of the different embryos analyzed.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/5/10.1182_blood.v98.5.1281/5/h81711446003.jpeg?Expires=1722366089&Signature=DzZk1rlEvowlvmhZlOr~PUCrPwWf5TguHbBHfwAFbiMoMS3OgXTijIkZYn8F0Aqxh-qA1EAg6H7-Oe-H-j5tijyZ1aR84oGbWqpmYDx3Wv0cr1gZ99GaJHDbvR52QnsZfb9btE8B~MS-Ucllk2cbkYh1IQcj~16ANADlo4JQjKHsZqt7pRbPJZehbfuVpaaimBVW1kSYuJzAicxBF7woy~QOai7lpBe5r1fBhGiDULLSrYshi2C6zKNCaXnP42SZLI1qBETZPDxXtg3PbZgrBxIEVq0Kn9an35uH42pif60ZM7RkTeyqOE3q2gy7RgPL~5mRJ43WVj9JQUj80j3GsA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transgene expression.
Detection under dark field illumination (magnification × 6) of CMV-GMTag AU− (A) and AU+ (B) transgene expression in E14 embryo by in situ hybridization using [35S]-labeled GM-CSF antisense riboprobe; (C) nontransgenic embryo. The white dots observed in the CMV-GMTag AU− sample correspond to dispersed transgenic mRNA-containing cells. White lines appearing in the CMV-GMTag AU+ and nontransgenic samples correspond to artifacts due to dust and manipulation. (D-G) Detection under bright field illumination of CMV-GMTagAU− transgene expression (black dots) (D) and expression foci at greater magnification (× 100) in the choroid plexus (E), the olfactory epithelium (F), and heart (G). (H) Staining of the heart by hemalum-eosin to show transgene expression by cells in clusters in blood vessels (arrows); br indicates brain; cp, choroı̈d plexus; he, heart; oe, olfactory epithelium; and ve, ventricule. These expression patterns are representative of the different embryos analyzed.
