Fig. 2.
![Fig. 2. Analysis of GMTag protein biologic activity. / The GM-CSF/IL-3 proliferation-dependent NFS-60 cells were incubated with decreasing concentrations of nontransfected (○) or pBK-CMV–GMTAg AU− transfected (●) HeLa cell supernatants. The first dilution of the pBK-CMV–GMTag AU−transfected cell supernatant contained 10 ng/mL GM-CSF as determined by ELISA. (■) Cells were incubated with 100 μL supernatant of pBK-CMV–GMTAg AU− transfected HeLa cells in the presence of decreasing concentrations of antimouse GM-CSF antibody. The transfected cell supernatant contained 20 ng/mL GM-CSF as determined by ELISA. Cell proliferation was measured by [3H]-thymidine incorporation (see “Materials and methods”).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/5/10.1182_blood.v98.5.1281/5/h81711446002.jpeg?Expires=1722371804&Signature=CEp0~SjAM4Qicftc5DQpfsHvitFB1-FqSwxskN96SmjonsRO31LcR46phdTEutjx1wVGXMnXaTdq3B29LfTNNaiC8GhaVLXQdXOv1Y0chmEel7X94YHB5THJS61dxQCBT-PUVr7OvQ4I~bWInlezoW~EbD2QKuX6Jq8BigXQcClTiTLmU1Btk3z7rsaEbBemAr2oHCbnoeonzZUxpb8iuko6q6xORAIYnoA20NTe94uMG0DHUucIm2irzmETuB0zV-LDB-3Ly9UECF639iYDd~whVaniMihW2J5BYhiEWGifywA8uZBeYv-KF0gyvaDhIn8LlYcSTieVQLV5~oQjwA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Analysis of GMTag protein biologic activity.
The GM-CSF/IL-3 proliferation-dependent NFS-60 cells were incubated with decreasing concentrations of nontransfected (○) or pBK-CMV–GMTAg AU− transfected (●) HeLa cell supernatants. The first dilution of the pBK-CMV–GMTag AU−transfected cell supernatant contained 10 ng/mL GM-CSF as determined by ELISA. (■) Cells were incubated with 100 μL supernatant of pBK-CMV–GMTAg AU− transfected HeLa cells in the presence of decreasing concentrations of antimouse GM-CSF antibody. The transfected cell supernatant contained 20 ng/mL GM-CSF as determined by ELISA. Cell proliferation was measured by [3H]-thymidine incorporation (see “Materials and methods”).
