Fig. 1.
Fig. 1. Effect of P-selectin antibodies on the adherence of nonsickle and sickle erythrocytes to HUVEC treated with or without thrombin. / The data shown are the static adherence of RBCs to HUVECs that were treated with thrombin or medium alone and then exposed to medium with or without blocking P-selectin antibody 9E1. The 100% adherence is the mean number of nonsickle (AA) RBC/field adherent to untreated HUVECs. SS, sickle erythorocytes. (A) The reduction of erythrocyte adherence to untreated or thrombin-treated HUVECs in the presence () or absence (■) of mAb 9E1. The data are mean percent adherence from 12 replicate experiments. Significant inhibition of adherence due to mAb 9E1 with P < .05 is denoted by *. (B) Titration of adhesion inhibition by mAb 9E1. The data from this experiment are mean percent adherence plotted versus the dilution of mAb 9E1. ▴, AA, −thrombin; ○, AA, +thrombin; ♦, SS, −thrombin; ■, SS, +thrombin.

Effect of P-selectin antibodies on the adherence of nonsickle and sickle erythrocytes to HUVEC treated with or without thrombin.

The data shown are the static adherence of RBCs to HUVECs that were treated with thrombin or medium alone and then exposed to medium with or without blocking P-selectin antibody 9E1. The 100% adherence is the mean number of nonsickle (AA) RBC/field adherent to untreated HUVECs. SS, sickle erythorocytes. (A) The reduction of erythrocyte adherence to untreated or thrombin-treated HUVECs in the presence () or absence (■) of mAb 9E1. The data are mean percent adherence from 12 replicate experiments. Significant inhibition of adherence due to mAb 9E1 with P < .05 is denoted by *. (B) Titration of adhesion inhibition by mAb 9E1. The data from this experiment are mean percent adherence plotted versus the dilution of mAb 9E1. ▴, AA, −thrombin; ○, AA, +thrombin; ♦, SS, −thrombin; ■, SS, +thrombin.

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