Induction of apoptosis by γ-irradiation is selectively reduced in Bcr-Abl⁺ BaF3 cells following adhesion to fibronectin.

(A) Bcr-Abl⁺ BaF3 cells with or without STI571/IL-3 and Bcr-Abl⁻ wtBaF3 cells were cultured for 24 hours on immobilized fibronectin (▪) or polylysin (░). Selectively adherent cells were then γ-irradiated with indicated dosages. After a growing period of 17 hours, the percentage of apoptotic cells was determined by TUNEL-assay. Values reflect the mean ± SEM of 3 independent examinations. (B) Percentage of apoptotic cells following irradiation with 0 Gy and 50 Gy of bcr-abl–transfected 32D (left panel) and Mig-transfected 32D cells (right panel). Apoptosis was detected with Annexin V-PE. Values reflect the mean ± SEM of 3 independent examinations. ▪ indicates fibronectin; ░, polylysin.