Inhibitory effects of HAGBPs on proliferation of the K562 CML blast cell line.

Proliferation without peptide addition was set to 100%. Peptides were added as detailed in Materials and methods. Continuous peptides without disulfide bond (P3-P5) were effective only above 50 μM. Therefore, concentrations below 30 μM are not shown here. Disulfide-bridged peptides were much more potent and therefore extensively tested in a concentration range between 0.5 and 20 μM as shown. Error bars indicate SEM (n = 6 to 9). ■ indicates P7 (Antp-SS-Ctrl GGLL); ▵, P6 (Antp-SS-HAGBP); ▪, P3 (Antp); ✖, P5 (Antp-Ctrl LL); and ▴, P4 (Antp-HAGBP).