Fig. 6.
PKB activation in fMLP, ANCA, and FcγR cross-linked neutrophils.
Phospho-PKB (pPKB) levels were assessed in blots of PKB immunoprecipitates from primed neutrophils stimulated with either (A) 1 μM fMLP, 250 μg/mL MPO-ANCA, PR3-ANCA, or normal IgG or (B) 1 μg/mL anti-FcγRII, 1 μg/mL anti-FcγRIII, or 1 μg/mL of both anti-FcγR monoclonal antibodies followed by 10 μg/mL GAM F(ab′)2 cross-linking antibody for 30 seconds, 1 minute, and 15 minutes. Molecular weight (kd), as assessed using Rainbow markers (Amersham), are shown on the left. Levels of PKB are also shown to indicate equal amounts of protein within the samples. These results are representative of 3 independent experiments.
