Fig. 4.
Effect of LY294002 on fMLP, ANCA, and anti-FcγR superoxide production.
Aliquots of 105 neutrophils were primed with 2 ng/mL TNF-α and treated with 0.5 (■), 5 (░), 10 (▨), or 50 μM LY294002 (▥) before stimulation with (A) 1 μM fMLP, 200 μg/mL MPO-ANCA, or PR3-ANCA IgG or (B) 1 μg/mL IV.3 (FcRII XL), 1 μg/mL 3G8 (FcRIII XL), or 1 μg/mL of both anti-FcγR monoclonal antibodies (FcRII+III XL) followed by 10 μg/mL GAM F(ab′)2cross-linking antibody for 15 minutes. All experiments were repeated 3 times, with 6 replicates of all samples. Results are shown as mean percentage inhibition of superoxide production ± SEM of data pooled from all 3 experiments.
