Fig. 2.
Effect of FcγR blocking on ANCA IgG superoxide response.
Aliquots of 105 neutrophils were primed with 2 ng/mL TNF-α and pretreated with either 1 μg/mL IV.3 (anti-FcRII, ■), 1 μg/mL 3G8 (anti-FcRIII, ░), or both monoclonal antibodies (anti-FcRII+III, ▨) before stimulation with 200 μg/mL MPO-ANCA or PR3-ANCA IgG for 15 minutes. All experiments were repeated 3 times using neutrophils from different donors, 3 different MPO-ANCA and PR3-ANCA preparations, and 6 replicates of all samples. Results show mean ± SEM of data pooled from all 3 experiments.
