Quality control of E-chip.

(A) Image of a laser-scanned microarray hybridized with 50 μg Cy3- and Cy5-labeled probe from the same pool of K562 erythroleukemia cell total RNA. (B) Scatter plot of signal intensity data collected from NBM (Cy3, 550-nm) scan and K562 (Cy5, 650-nm) scan with 3 SDs. (C) Density plot of NBM (Cy3) signal intensity over K562 (Cy5) signal intensity. The ratio limits (Cy3/Cy5 ratios of less than 0.85 or greater than 1.43) used to calculate significant changes in expression levels indicate P ≤ .01.