Fig. 1.
Fig. 1. Abrogation of proliferative allo-antigen and mitogen responses of murine splenocytes by the JAK3 inhibitor WHI-P131. / The effects of WHI-P131 on allo-antigen and mitogen responses of JAK3+/+ splenocytes from WT C57BL/6 mice were examined in MLR (A), PHA (B), and ConA (C) assays, as described in “Materials and methods.” WHI-P131 was applied at concentrations of 0 (vehicle alone), 0.1, 1, 10, and 50 μg/mL during the 5-day (MLR) or 3-day culture period (PHA and ConA assays). Proliferation was measured using the colorimetric WST-1 assay. Results are presented as mean OD (± SEM) values from 3 to 6 independent experiments. Statistical differences between the WHI-P131 treatment groups and vehicle-treated control groups were analyzed by Student ttests: *P < .05; **P < .0005; and ***P < .0001.

Abrogation of proliferative allo-antigen and mitogen responses of murine splenocytes by the JAK3 inhibitor WHI-P131.

The effects of WHI-P131 on allo-antigen and mitogen responses of JAK3+/+ splenocytes from WT C57BL/6 mice were examined in MLR (A), PHA (B), and ConA (C) assays, as described in “Materials and methods.” WHI-P131 was applied at concentrations of 0 (vehicle alone), 0.1, 1, 10, and 50 μg/mL during the 5-day (MLR) or 3-day culture period (PHA and ConA assays). Proliferation was measured using the colorimetric WST-1 assay. Results are presented as mean OD (± SEM) values from 3 to 6 independent experiments. Statistical differences between the WHI-P131 treatment groups and vehicle-treated control groups were analyzed by Student ttests: *P < .05; **P < .0005; and ***P < .0001.

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