Zebrafish myelopoietic cytomorphology.

Myelopoietic cells detected by various histological and cytochemical methods. Low (A) and high (B) magnification micrographs of adult zebrafish kidney tissue sections stained with hematoxylin and eosin. Micrographs of adult zebrafish kidney smears (C,G-J) and cytospins (D-F), stained with Wright-Giemsa stain (C-F), MPO (G), demonstrating the neutrophil cytoplasm (brown precipitate, black arrowhead), and acid phosphatase showing reactivity with neutrophils (H; red stain, black arrowhead). PAS specifically stained basophils/eosinophils (I; bright pink stain, black arrowhead), and double staining with MPO and PAS (J) showed distinct neutrophils and basophils/eosinophils, respectively. T = renal tubule; Bp = basophil/eosinophil promyelocyte; E = erythrocyte; N = neutrophil; pm = promyelocyte; B = basophil/eosinophil; Me = metamyelocyte; Ba = band. (A) 50 ×; (B) 150 ×; (C,G-J) 250 ×; (D-F) 300 ×.