Fig. 3.
Ro 31-9790 arrests lymphocytes in the walls of HEVs and prevents entry into LNs.
The HEC lining HEVs in LN sections was stained with mAb MECA 79 and CFSE-labeled lymphocytes recorded as either inside HEVs or outside HEVs at 30, 60, and 120 minutes after injection. Representative images showing the localization of CFSE-labeled lymphocytes inside (arrows) and outside HEVs (arrowheads) in control and Ro 31-9790-treated mice are shown. Histograms show mean number of CFSE-labeled lymphocytes/mm2 ± SEM pooled from inguinal and brachial LNs of 9 (30 and 60 minutes) or 3 mice (120 minutes) treated with Ro 31-9790 (solid bars) or vehicle (open bars). **P < .005; *P < .05. Bar indicates 50 μm.
