PMNs and Kupffer cells in the liver.

(A) Double staining showed that TUNEL-positive (red, arrows) and TUNEL-negative (blue, arrowheads) PMNs were trapped by ED2⁺Kupffer cells (brown). Both PMNs and Kupffer cells were labeled by Fas (B) and FasL (C) after LPS injection. (D) Number of phagocytosed cells peaked 6 hours after LPS injection; moreover, TUNEL-negative cells occupied more than 30% in phagocytosed cells. (E) Number of TUNEL-positive cells was reduced at 3 hours, and significant reduction was observed 6 hours after combined treatment with anti-Fas Ab, compared with LPS treatment alone. *P < .05 versus LPS treatment only. Scale bar, 20 μm.