Surface expression of L-selectin on granulocytes from wild-type (+/+) and triple-null (−/−) mice.

Peripheral blood was incubated with FITC-conjugated MEL-14 mAb and analyzed by flow cytometry. The fluorescence histogram is representative of those from several mice (n = 6) from different litters on a 4-decade log scale. (A) Granulocytes from triple-null (−/−) mice overlaid on a granulocyte population incubated with an isotope-matched mAb. (B) Granulocytes from wild-type (+/+) mice stained with FITC-labeled MEL-14 mAb produced a pronounced shift in fluorescence over background fluorescence.