Expression pattern of the

DUB-2A mRNA. Genomic PCR products and RT-PCR products were generated from the indicated cell lines, by means of degenerate primers and DUB-2A-specific primers. The PCR products were electrophoresed on a 1% agarose gel, which was stained with ethidium bromide. The DUB-2A mRNA was detected by RT-PCR in several murine cell lines. including CTLL (T cells), 32D (myeloid cells), and ES cells, but not in murine F9 carcinoma cell lines or NIH3T3 fibroblasts. A DUB mRNA was detected by RT-PCR from all cell lines when degenerate DUB primers were used (lanes 3 though 7).