Effect of Der p 1–pulsed DCs on IL-4 and IFN-γ production by T cells.

Monocyte-derived DCs from house dust mite–sensitive patients (A,B), from grass pollen–sensitive patients (C,D), and from healthy donors (E,F) were pulsed or not with 100 ng/mL Der p 1 or ProDer p 1 for 24 hours. Then, Der p 1–pulsed or unpulsed DCs were cocultured with autologous T cells for 24 and 48 hours. Supernatants were harvested, and the amounts of IL-4 and IFN-γ were measured by specific ELISA. ░, without Der p 1; ▪, with Der p 1; ■, with ProDer p 1. Results are expressed as the mean ± SEM. *P < .05.