American Society of Hematology

Fig. 3.

$Fig. 3. Effect of PGE1 and NEM treatment on calmodulin association with GPIb-IX. / (A) Triton X-100 soluble fraction of platelets either untreated or treated with 3 nM PGE1 for 5 minutes at room temperature were immunoprecipitated by means of nonimmune sera (NonI) or GPIb antibodies and were immunoblotted by means of antibodies to calmodulin. (B) Triton X-100 soluble fractions of platelets either untreated or treated with 1 mM NEM for 5 minutes at room temperature were immunoprecipitated by means of nonimmune sera (NonI) or GPIb antibodies and were immunoblotted by means of antibodies to actin-binding protein (ABP, upper panel) or calmodulin (lower panel). Blots were visualized by means of a peroxidase-coupled second antibody and the ECL reagent. Results are representative of 3 separate experiments.$

Effect of PGE₁ and NEM treatment on calmodulin association with GPIb-IX.

(A) Triton X-100 soluble fraction of platelets either untreated or treated with 3 nM PGE₁ for 5 minutes at room temperature were immunoprecipitated by means of nonimmune sera (NonI) or GPIb antibodies and were immunoblotted by means of antibodies to calmodulin. (B) Triton X-100 soluble fractions of platelets either untreated or treated with 1 mM NEM for 5 minutes at room temperature were immunoprecipitated by means of nonimmune sera (NonI) or GPIb antibodies and were immunoblotted by means of antibodies to actin-binding protein (ABP, upper panel) or calmodulin (lower panel). Blots were visualized by means of a peroxidase-coupled second antibody and the ECL reagent. Results are representative of 3 separate experiments.

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