Translocation of platelet proteins to the activated cytoskeleton.

Platelets that were untreated or stimulated with α-thrombin (1 U/mL, final concentration) for 5 minutes at room temperature were lysed, and cytosolic and actin cytoskeletal (CYSK) fractions were isolated and immunoblotted with antibodies to p85, calmodulin, GPIbα, or GPV. Blots were visualized by means of a peroxidase-coupled second antibody and the ECL reagent. Results are typical of 3 separate experiments.