Representative standard curve for quantifying BCL-6 gene expression in tumor specimens.

Human RNA is diluted 5-fold in 5 increments (from 50 ng to 0.08 ng) and subjected to real-time quantitative RT-PCR in triplicate. RNA input of standards is plotted against threshold cycle (Ct) to create a standard curve for quantitation of the BCL-6 mRNA of unknown tumor samples.