Fig. 2.
Fig. 2. Morphology of day 7 colonies derived from sorted FL myeloid progenitors. / Two hundred cells from each population were cultured in methylcellulose containing SLF, Flt-3 L, IL-3, IL-6, IL-11, EPO, TPO, and FL-conditioned media in 35-mm dishes. Upper panels (A-C) show the appearance of colonies in methylcellulose, and lower panels (D-F) show the cellular morphologies of colonies pooled from each progenitor subset (Giemsa/May-Grünwald staining). All types of myeloerythroid colonies were generated from FcγRloCD34+ cells (A, D). CFU-MegE colonies were generated from FcγRloCD34− cells (B, E), and CFU-GM, CFU-M, and CFU-G colonies were generated from FcγRhiCD34+ cells (C, F). Relative magnifications for each panel were (A) 8×, (B) 40×, (C) 8×, (D) 20×, (E) 60×, and (F) 40×.

Morphology of day 7 colonies derived from sorted FL myeloid progenitors.

Two hundred cells from each population were cultured in methylcellulose containing SLF, Flt-3 L, IL-3, IL-6, IL-11, EPO, TPO, and FL-conditioned media in 35-mm dishes. Upper panels (A-C) show the appearance of colonies in methylcellulose, and lower panels (D-F) show the cellular morphologies of colonies pooled from each progenitor subset (Giemsa/May-Grünwald staining). All types of myeloerythroid colonies were generated from FcγRloCD34+ cells (A, D). CFU-MegE colonies were generated from FcγRloCD34− cells (B, E), and CFU-GM, CFU-M, and CFU-G colonies were generated from FcγRhiCD34+ cells (C, F). Relative magnifications for each panel were (A) 8×, (B) 40×, (C) 8×, (D) 20×, (E) 60×, and (F) 40×.

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